Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | acts as a powerful allosteric effector on the methyltransferase reaction. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase | Methanosarcina barkeri | |
methyltransferase activating protein | MAP, dependent on. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase | Methanosarcina barkeri | |
additional information | as isolated, DMA-MT is inactive, but it can enzymically be reactivated by methyltransferase activating protein, ATP, and hydrogenase | Methanosarcina barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Monomethylamine | competitive inhibitor | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Methanosarcina barkeri |
100000 | - |
gel filtration | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri Fusaro / DSM 804 | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
DSM 804 | - |
Methanosarcina barkeri Fusaro / DSM 804 | - |
DSM 804 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 62.8fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and anion exchange chromatography | Methanosarcina barkeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:trimethylamine-grown cell | - |
Methanosarcina barkeri | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | methylation of the corrinoid prosthetic group | Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri Fusaro / DSM 804 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | methylation of the corrinoid prosthetic group | Methanosarcina barkeri Fusaro / DSM 804 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
dimethyl-12-HBI methyltransferase | - |
Methanosarcina barkeri |
dimethylamine/5-hydroxybenzimidazolylcobamide methyltransferase | - |
Methanosarcina barkeri |
DMA-MT | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
- |
Methanosarcina barkeri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 46 | rapid decline of activity above 46°C, inactive at 55°C | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
corrinoid protein | prosthetic group, with a corrinoid content of 0.9 mol B12/mol holoenzyme | Methanosarcina barkeri |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
Monomethylamine | pH 7.0, 37°C | Methanosarcina barkeri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanosarcina barkeri | isoelectric focusing | - |
4.7 |
General Information | Comment | Organism |
---|---|---|
additional information | as-isolated DMA-MT is inactive. The enzyme can be reactivated to yield the methylated B12-HBI prosthetic group by a system that comprises a methyl donor, a reductant, ATP and one (or more) component(s) present in the partially purified methyltransferase activating protein, MAP, fraction | Methanosarcina barkeri |